CD130 Antibody (C-term)

APR03528G Product Datasheet Protocol

貨號	貨期	規(guī)格 / 價格	詢價
APR03528G		100 μl / ￥4950

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安諾倫（北京）生物科技有限公司

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CD130 Antibody (C-term)

品牌	Leading Biology	貨號	APR03528G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality CD130 Antibody (C-term).
分子量	103537 Da
細(xì)胞定位	Antigen Cellular Localization: Isoform 1: Cell membrane; Single-pass type I membrane protein
宿主	Rabbit
種屬反應(yīng)性	Human
免疫原	871-899 aa
靶點	This CD130 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 871-899 amino acids from the C-terminal region of human CD130.
亞型	Rabbit Ig
基因ID	3572
UniProt ID	P40189
功能	Signal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp130 for initiating signal transmission. Binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. Does not bind IL6. May have a role in embryonic development (By similarity). The type I OSM receptor is capable of transducing OSM-specific signaling events.
總結(jié)	The protein encoded by this gene is a signal transducershared by many cytokines, including interleukin 6 (IL6), ciliaryneurotrophic factor (CNTF), leukemia inhibitory factor (LIF), andoncostatin M (OSM). This protein functions as a part of thecytokine receptor complex. The activation of this protein isdependent upon the binding of cytokines to their receptors. vIL6, aprotein related to IL6 and encoded by the Kaposi sarcoma-associatedherpesvirus, can bypass the interleukin 6 receptor (IL6R) anddirectly activate this protein. Knockout studies in mice suggestthat this gene plays a critical role in regulating myocyteapoptosis. Alternatively spliced transcript variants encodingdistinct isoforms have been described. A related pseudogene hasbeen identified on chromosome 17.
儲存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, IHC-P, E
稀釋方法	WB~~1:1000 IHC-P~~1:50~100
圖像	All lanes : Anti-CD130 Antibody (C-term) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: MDA-MB-231 whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: SW480 whole cell lysate Lane 5: A549 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 104 kDa Blocking/Dilution buffer: 5% NFDM/TBST. All lanes : Anti-CD130 Antibody (C-term) at 1:2000 dilution Lane 1: A549 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: HT-1080 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size :104kDa Blocking/Dilution buffer: 5% NFDM/TBST. APR03528G staining CD130 in Human skeletal muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
說明書	Product Datasheet Protocol
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