品牌 |
Leading Biology | 貨號 |
APR06209G |
產(chǎn)品分類 |
Polyclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality GTSE1 Antibody (C-term).
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分子量 |
76645 Da
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細(xì)胞定位 |
Antigen Cellular Localization:
Cytoplasm, cytoskeleton. Note=Associated with microtubules
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human
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免疫原 |
690-717 aa
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靶點 |
This GTSE1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 690-717 amino acids from the C-terminal region of human GTSE1.
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亞型 |
Rabbit Ig
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基因ID |
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UniProt ID |
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功能 |
May be involved in p53-induced cell cycle arrest in G2/M phase by interfering with microtubule rearrangements that are required to enter mitosis. Overexpression delays G2/M phase progression.
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總結(jié) |
GTSE1 is only expressed in the S and G2 phases of the cell cycle, where it colocalizes with cytoplasmic tubulin and microtubules. In response to DNA damage, the encoded protein accumulates in the nucleus and binds the tumor suppressor protein p53, shuttling it out of the nucleus and repressing its ability to induce apoptosis.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, IHC-P, FC, E
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稀釋方法 |
WB~~1:1000
IHC-P~~1:10~50
FC~~1:10~50
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圖像 |
All lanes : Anti-GTSE1 Antibody (C-term) at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: MCF-7 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 77 kDa Blocking/Dilution buffer: 5% NFDM/TBST. GTSE1 Antibody (C-term) (Cat. APR06209G) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GTSE1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated. GTSE1 Antibody (C-term) (Cat. APR06209G) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
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說明書 |
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數(shù)量 |
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