品牌 |
Leading Biology | 貨號 |
APR06171G |
產品分類 |
Polyclonal Antibodies | 研究領域 |
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產品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality IL2RG Antibody (N-term).
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分子量 |
42287 Da
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細胞定位 |
Antigen Cellular Localization:
Membrane; Single-pass type I membrane protein
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宿主 |
Rabbit
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種屬反應性 |
Human
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免疫原 |
76-101 aa
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靶點 |
This IL2RG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 76-101 amino acids from the N-terminal region of human IL2RG.
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亞型 |
Rabbit Ig
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基因ID |
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UniProt ID |
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功能 |
Common subunit for the receptors for a variety of interleukins.
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總結 |
IL2RG is an important signaling component of many interleukin receptors, including those of interleukin -2, -4, -7 and -21, and is thus referred to as the common gamma chain. Mutations in this gene cause X-linked severe combined immunodeficiency (XSCID), as well as X-linked combined immunodeficiency (XCID), a less severe immunodeficiency disorder.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應用 |
WB, IF, FC, E
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稀釋方法 |
IF~~1:25
WB~~1:1000
FC~~1:10~50
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圖像 |
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized human spleen tissue labeling Pdx1 with APR06171G at 1/25 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/400 dilution (green). Confocal image showing membrane staining on human spleen tissue. The nuclear counter stain is DAPI (blue). All lanes : Anti-IL2RG Antibody (N-term) at 1:2000 dilution Lane 1: human spleen lysates Lane 2: Jurkat whole cell lysates Lane 3: Daudi whole cell lysates Lane 4: KG-1 whole cell lysates Lane 5: MOLT-4 whole cell lysates Lane 6: HL-60 whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 42 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Western blot analysis of lysate from Jurkat cell line, using IL2RG Antibody (N-term)(APR06171G). APR06171G was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug. |
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說明書 |
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數(shù)量 |
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