NEK3 Antibody (Center)

APR06063G Product Datasheet Protocol

貨號	貨期	規(guī)格 / 價(jià)格	詢價(jià)
APR06063G		100 μl / ￥4950

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安諾倫（北京）生物科技有限公司

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NEK3 Antibody (Center)

品牌	Leading Biology	貨號	APR06063G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality NEK3 Antibody (Center).
分子量	57705 Da
細(xì)胞定位	Antigen Cellular Localization: Cytoplasm. Cell projection, axon
宿主	Rabbit
種屬反應(yīng)性	Human
免疫原	331-361 aa
靶點(diǎn)	This NEK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 331-361 amino acids from the Central region of human NEK3.
亞型	Rabbit Ig
基因ID	4752
UniProt ID	P51956
功能	Protein kinase which influences neuronal morphogenesis and polarity through effects on microtubules. Regulates microtubule acetylation in neurons. Contributes to prolactin- mediated phosphorylation of PXN and VAV2. Implicated in prolactin- mediated cytoskeletal reorganization and motility of breast cancer cells through mechanisms involving RAC1 activation and phosphorylation of PXN and VAV2.
總結(jié)	In Aspergillus nidulans, lack of the serine/threonine kinase NimA (never in mitosis A) results in cell cycle arrest in G2, while overexpression causes the premature onset of mitotic events. NEK3 is similar in sequence to the Aspergillus nidulans protein and may therefore play a role in mitotic regulation. However, the encoded protein differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm.
儲存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, E
稀釋方法	WB~~1:1000
圖像	All lanes : Anti-NEK3 Antibody (K346) at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: CCRF-CEM whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 58 kDa Blocking/Dilution buffer: 5% NFDM/TBST. All lanes : Anti-NEK3 Antibody (K346) at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: SH-SY5Y whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 58 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Western blot analysis of NEK3 (arrow) using rabbit polyclonal NEK3 Antibody (K346) (Cat. APR06063G). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NEK3 gene.
說明書	Product Datasheet Protocol
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