品牌 |
Leading Biology |
貨號(hào) |
APR05890G |
產(chǎn)品分類 |
Polyclonal Antibodies |
研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality CPN1 Antibody (N-term).
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分子量 |
52286 Da
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細(xì)胞定位 |
Antigen Cellular Localization:
Secreted, extracellular space.
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human, Mouse
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免疫原 |
52-81 aa
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靶點(diǎn) |
This CPN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 52-81 amino acids from the N-terminal region of human CPN1.
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亞型 |
Rabbit Ig
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通用名 |
ACBP
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基因ID |
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UniProt ID |
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功能 |
Protects the body from potent vasoactive and inflammatory peptides containing C-terminal Arg or Lys (such as kinins or anaphylatoxins) which are released into the circulation.
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總結(jié) |
CPN1 is a plasma metallo-protease that cleaves basic amino acids from the C terminal of peptides and proteins. The protein is important in the regulation of peptides like kinins and anaphylatoxins, and has also been known as kininase-1 and anaphylatoxin inactivator. This protein is a tetramer comprised of two identical regulatory subunits and two identical catalytic subunits.
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儲(chǔ)存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, E
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稀釋方法 |
WB~~1:1000
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圖像 |
All lanes : Anti-CPN1 Antibody (N-term) at 1:1000 dilution
Lane 1: human liver lysate
Lane 2: HepG2 whole cell lysate
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 52 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot analysis of CPN1 antibody (N-term) (Cat.APR05890G) in K562, CEM, HEpG2 cell line lysates and mouse liver tissue lysates (35ug/lane). CPN1(arrow) was detected using the purified Pab.
Western blot analysis of CPN1 (arrow) using rabbit polyclonal CPN1 Antibody (N-term) (Cat. APR05890G). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CPN1 gene.
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說(shuō)明書(shū) |
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數(shù)量 |
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