CAMK1D (CAMK1 delta)Antibody (C-term)

APR05854G Product Datasheet Protocol

貨號	貨期	規(guī)格 / 價格	詢價
APR05854G		100 μl / ￥4950

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CAMK1D (CAMK1 delta)Antibody (C-term)

品牌	Leading Biology	貨號	APR05854G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality CAMK1D (CAMK1 delta)Antibody (C-term).
分子量	42914 Da
細(xì)胞定位	Antigen Cellular Localization: Cytoplasm. Nucleus. Note=Predominantly cytoplasmic. Nuclear localization increases upon activation by KCl treatment in hippocampal neurons
宿主	Rabbit
種屬反應(yīng)性	Human
免疫原	302-333 aa
靶點	This CAMK1D (CAMK1 delta) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 302-333 amino acids from the C-terminal region of human CAMK1D (CAMK1 delta).
亞型	Rabbit Ig
通用名	CAMKID
基因ID	57118
UniProt ID	Q8IU85
功能	Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK1 signaling cascade and, upon calcium influx, activates CREB-dependent gene transcription, regulates calcium-mediated granulocyte function and respiratory burst and promotes basal dendritic growth of hippocampal neurons. In neutrophil cells, required for cytokine- induced proliferative responses and activation of the respiratory burst. Activates the transcription factor CREB1 in hippocampal neuron nuclei. May play a role in apoptosis of erythroleukemia cells. In vitro, phosphorylates transcription factor CREM isoform Beta.
總結(jié)	CAMK1-like protein kinase belongs to a proposed calcium-triggered signaling cascade. This protein is expressed in polymorphonuclear leukocytes (PMNs) and may be part of the chemokine signal transduction pathway that regulates granulocyte function. CAMK1-like protein kinase may play a role in apoptosis of erythroleukemia cells. It activates MAP kinase MAPK3, and in vitro, phosphorylates transcription factor CREM isoform Beta and probably CREB1.
儲存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, IHC-P, E
稀釋方法	WB~~1:1000 IHC-P~~1:50~100
圖像	Western blot analysis of anti-CAMK1-like C-term Pab (Cat. APR05854G) in NCIH460 cell lysate. CAMK1-like (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. Western blot analysis of CAMK1D (arrow) using CAMK1D (CAMK1 delta)Antibody (C-term) (Cat. APR05854G). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CAMK1D gene (Lane 2). Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
說明書	Product Datasheet Protocol
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