KIST (KIS) Antibody (C-term)

APR05840G Product Datasheet Protocol

貨號(hào)	貨期	規(guī)格 / 價(jià)格	詢價(jià)
APR05840G		100 μl / ￥4950

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安諾倫（北京）生物科技有限公司

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KIST (KIS) Antibody (C-term)

品牌	Leading Biology	貨號(hào)	APR05840G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality KIST (KIS) Antibody (C-term).
分子量	46546 Da
細(xì)胞定位	Antigen Cellular Localization: Nucleus.
宿主	Rabbit
種屬反應(yīng)性	Human, Mouse
免疫原	265-295 aa
靶點(diǎn)	This KIST (KIS) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 265-295 amino acids from the C-terminal region of human KIST (KIS).
亞型	Rabbit Ig
通用名	KIS, KIST
基因ID	127933
UniProt ID	Q8TAS1
功能	Upon serum stimulation, phosphorylates CDKN1B/p27Kip1, thus controlling CDKN1B subcellular location and cell cycle progression in G1 phase. May be involved in trafficking and/or processing of RNA (By similarity).
總結(jié)	KIST, a member of the Ser/Thr protein kinase family, is a pyruvate kinase that catalyzes formation of phosphoenolpyruvate from pyruvate and ATP. A role for the primarily nuclear KIST protein in mediation of cellular metabolism has been postulated based on the interaction identified with thyroid hormone. KIST is widely expressed, with highest abundance in skeletal muscle, kidney, placenta and peripheral blood leukocytes. Upon serum stimulation, KIST phosphorylates CDKN1B/p27Kip1, thereby regulating the subcellular location of CDKN1B and cell cycle progression in the G1 phase. KIST, which contains one RNA recognition motif domain, has been proposed to partipate in trafficking and processing of RNA. KIST binds to Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells.
儲(chǔ)存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, E
稀釋方法	WB~~1:1000
圖像	All lanes : Anti-KIST Antibody (L280) at 1:1000 dilution Lane 1: human skeletal muscle lysate Lane 2: A-673 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 47 kDa Blocking/Dilution buffer: 5% NFDM/TBST. The anti-KIST Pab (Cat. APR05840G) is used in Western blot to detect KIST in K562 cell lysate (Lane 1) and mouse liver tissue lysate (Lane 2).
說明書	Product Datasheet Protocol
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