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當(dāng)前位置：首頁(yè) > 抗原抗體、ELISA、WB > 一抗 > Polyclonal Antibodies > ALAD Antibody (C-term)

ALAD Antibody (C-term)

貨號(hào) 貨期 規(guī)格 / 價(jià)格 詢價(jià)
APR05809G 100 μl / ￥4950

ALAD Antibody (C-term)

品牌

 Leading Biology

貨號(hào)

 APR05809G

產(chǎn)品分類

 Polyclonal Antibodies

研究領(lǐng)域

產(chǎn)品概述
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality ALAD Antibody (C-term).

分子量
36295 Da

宿主
Rabbit

種屬反應(yīng)性
Human

免疫原
244-272 aa

靶點(diǎn)
This ALAD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 244-272 amino acids from the C-terminal region of human ALAD.

亞型
Rabbit Ig

基因ID

UniProt ID

功能
Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen.

總結(jié)
The ALAD enzyme is composed of 8 identical subunits and catalyzes the condensation of 2 molecules of delta-aminolevulinate to form porphobilinogen (a precursor of heme, cytochromes and other hemoproteins). ALAD catalyzes the second step in the porphyrin and heme biosynthetic pathway; zinc is essential for enzymatic activity. ALAD enzymatic activity is inhibited by lead and a defect in the ALAD structural gene can cause increased sensitivity to lead poisoning and acute hepatic porphyria.

儲(chǔ)存條件
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

應(yīng)用
WB, IHC-P, FC, E

稀釋方法
WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50

圖像

Western blot analysis of ALAD Antibody (C-term) (Cat. APR05809G) in Jurkat cell line lysates (35ug/lane). ALAD (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human brain tissue reacted with ALAD Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

ALAD Antibody (C-term) (Cat. APR05809G) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

說(shuō)明書

數(shù)量

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