TRAP Antibody (N-term)

APR05766G Product Datasheet Protocol

貨號	貨期	規(guī)格 / 價格	詢價
APR05766G		100 μl / ￥4950

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品牌	Leading Biology	貨號	APR05766G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality TRAP Antibody (N-term).
分子量	29274 Da
細(xì)胞定位	Antigen Cellular Localization: Cell membrane; Single-pass type II membrane protein
宿主	Rabbit
種屬反應(yīng)性	Human
免疫原	33-62 aa
靶點	This TRAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 33-62 amino acids from the N-terminal region of human TRAP.
亞型	Rabbit Ig
通用名	CD40L, TNFSF5, TRAP
基因ID	959
UniProt ID	P29965
功能	Mediates B-cell proliferation in the absence of co- stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching.
總結(jié)	TRAP is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in its gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome.
儲存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, IF, IHC-P, FC, E
稀釋方法	WB~~1:1000 IHC-P~~1:10~50 IF~~1:10~50 FC~~1:10~50
圖像	All lanes : Anti-TRAP Antibody (N-term) at 1:1000 dilution Lane 1: Jurkat whole cell lysate Lane 2: THP-1 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 29 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Western blot analysis of TRAP antibody (N-term) (Cat. APR05766G) in NCI-H460 cell line lysates (35ug/lane). TRAP (arrow) was detected using the purified Pab. TRAP Antibody (N-term) (Cat. APR05766G) IHC analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TRAP Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
說明書	Product Datasheet Protocol
數(shù)量

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