PXN Antibody (Y118)

APR05754G Product Datasheet Protocol

貨號(hào)	貨期	規(guī)格 / 價(jià)格	詢價(jià)
APR05754G		100 μl / ￥4950

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安諾倫（北京）生物科技有限公司

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PXN Antibody (Y118)

品牌	Leading Biology	貨號(hào)	APR05754G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality PXN Antibody (Y118).
分子量	64505 Da
細(xì)胞定位	Antigen Cellular Localization: Cytoplasm, cytoskeleton. Cell junction, focal adhesion. Cytoplasm, cell cortex. Note=Colocalizes with integrins at the cell periphery.
宿主	Rabbit
種屬反應(yīng)性	Human
免疫原	94-125 aa
靶點(diǎn)	This PXN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 94-125 amino acids from human PXN.
亞型	Rabbit Ig
基因ID	5829
UniProt ID	P49023
功能	Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
總結(jié)	PXN is a cytoskeletal adapter protein involved in organisation and function of focal adhesions, which are critical to cell adhesion and migration. This in turn plays a role in a wide variety of processes including embryogenesis, organogenesis, wound repair, inflammation and cancer. PXN contains LD motifs, LIM domains, SH3 and SH2 binding domains that serve as docking sites for cytoskeletal proteins, tyrosine kinases (e.g., FAK, Pyk 2, Src), serine/threonine kinases, GTPase activating proteins and other adaptor proteins (e.g., Actin, Vinculin, Crk).
儲(chǔ)存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, IHC-P, E
稀釋方法	WB~~1:1000 IHC-P~~1:10~50
圖像	All lanes : Anti-PXN Antibody (Y118) at 1:1000 dilution Lane 1: A431 whole cell lysate Lane 2: MCF-7 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 65 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Western blot analysis of PXN(arrow) using rabbit polyclonal PXN Antibody (Y118) (Cat.APR05754G). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with PXN gene (Lane 2) (Origene Technologies). Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with PXN Antibody (Y118) (Cat.APR05754G), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
說明書	Product Datasheet Protocol
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