IL1R Antibody (C-term E487)

APR05750G Product Datasheet Protocol

貨號(hào)	貨期	規(guī)格 / 價(jià)格	詢價(jià)
APR05750G		100 μl / ￥4950

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安諾倫（北京）生物科技有限公司

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IL1R Antibody (C-term E487)

品牌	Leading Biology	貨號(hào)	APR05750G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality IL1R Antibody (C-term E487).
分子量	65402 Da
細(xì)胞定位	Antigen Cellular Localization: Membrane; Single-pass type I membrane protein Cell membrane. Secreted
宿主	Rabbit
種屬反應(yīng)性	Human
免疫原	474-503 aa
靶點(diǎn)	This IL1R antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 474-503 amino acids from the C-terminal region of human IL1R.
亞型	Rabbit Ig
通用名	IL1R, IL1RA, IL1RT1
基因ID	3554
UniProt ID	P14778
功能	Receptor for IL1A, IL1B and IL1RN. After binding to interleukin-1 associates with the corecptor IL1RAP to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B, MAPK and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Binds ligands with comparable affinity and binding of antagonist IL1RN prevents association with IL1RAP to form a signaling complex.
總結(jié)	IL1R is a member of the interleukin 1 receptor family. An experiment with transient gene expression demonstrated that this receptor was incapable of binding to interleukin 1 alpha and interleukin 1 beta with high affinity. IL1R is a receptor for interleukin 1 family member 9 (IL1F9). Binding to the agonist leads to the activation of NF-kappa-B. The gene for this protein and four other interleukin 1 receptor family genes, including interleukin 1 receptor, type I (IL1R1), interleukin 1 receptor, type II (IL1R2), interleukin 1 receptor-like 1 (IL1RL1), and interleukin 18 receptor 1 (IL18R1), form a cytokine receptor gene cluster in a region mapped to chromosome 2q12.
儲(chǔ)存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, IHC-P, E
稀釋方法	WB~~1:1000 IHC-P~~1:10~50
圖像	Anti-IL1R Antibody (C-term E487) at 1:2000 dilution + human placenta lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 65 kDa Blocking/Dilution buffer: 5% NFDM/TBST. All lanes : Anti-IL1R Antibody (pY496) at 1:1000 dilution Lane 1: HepG2 whole cell lysate Lane 2: HL-60 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 65 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with IL1R antibody (C-term E489), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
說明書	Product Datasheet Protocol
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