All lanes : Anti-Cytokine IK Antibody at 1:1000 dilution
Lane 1: Hela whole cell lysates
Lane 2: THP-1 whole cell lysates
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution
Predicted band size : 66 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.