NASP Antibody (N-term)

APR05360G Product Datasheet Protocol

貨號	貨期	規(guī)格 / 價格	詢價
APR05360G		100 μl / ￥4950

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NASP Antibody (N-term)

品牌	Leading Biology	貨號	APR05360G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality NASP Antibody (N-term).
分子量	85238 Da
細胞定位	Antigen Cellular Localization: Cytoplasm. Nucleus.
宿主	Rabbit
種屬反應(yīng)性	Human
免疫原	152-180 aa
靶點	This NASP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 152-180 amino acids from the N-terminal region of human NASP.
亞型	Rabbit Ig
基因ID	4678
UniProt ID	P49321
功能	Required for DNA replication, normal cell cycle progression and cell proliferation. Forms a cytoplasmic complex with HSP90 and H1 linker histones and stimulates HSP90 ATPase activity. NASP and H1 histone are subsequently released from the complex and translocate to the nucleus where the histone is released for binding to DNA (By similarity).
總結(jié)	NASP encodes a H1 histone binding protein that is involved in transporting histones into the nucleus of dividing cells. Multiple isoforms are encoded by transcript variants of this gene. The somatic form is expressed in all mitotic cells, is localized to the nucleus, and is coupled to the cell cycle. The testicular form is expressed in embryonic tissues, tumor cells, and the testis. In male germ cells, this protein is localized to the cytoplasm of primary spermatocytes, the nucleus of spermatids, and the periacrosomal region of mature spermatozoa.
儲存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, FC, E
稀釋方法	WB~~1:1000 FC~~1:10~50
圖像	All lanes : Anti-NASP Antibody (N-term) at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: human testis lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 85 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Western blot analysis of NASP (arrow) using rabbit polyclonal NASP Antibody (N-term) (Cat. APR05360G). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NASP gene (Lane 2) . NASP Antibody (N-term) (Cat. APR05360G) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
說明書	Product Datasheet Protocol
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