CJ119 Antibody (N-term)

APR05350G Product Datasheet Protocol

貨號(hào)	貨期	規(guī)格 / 價(jià)格	詢價(jià)
APR05350G		100 μl / ￥4950

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CJ119 Antibody (N-term)

品牌	Leading Biology	貨號(hào)	APR05350G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality CJ119 Antibody (N-term).
分子量	72980 Da
細(xì)胞定位	Antigen Cellular Localization: Nucleus. Note=Associates with chromatin. Highly associated with chromatin in G1/S and S phases, reduced binding to chromatin in G2, and further decreased binding in early M phase. It then reassociates with chromatin in late M phase. Dissociates from chromatin later than component of the MCM complex
宿主	Rabbit
種屬反應(yīng)性	Human
免疫原	143-171 aa
靶點(diǎn)	This CJ119 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 143-171 amino acids from the N-terminal region of human CJ119.
亞型	Rabbit Ig
通用名	C10orf119
基因ID	79892
UniProt ID	Q9BTE3
功能	Associated component of the MCM complex that acts as a regulator of DNA replication. Binds to the MCM complex during late S phase and promotes the disassembly of the MCM complex from chromatin, thereby acting as a key regulator of pre-replication complex (pre-RC) unloading from replicated DNA. Can dissociate the MCM complex without addition of ATP; probably acts by destabilizing interactions of each individual subunits of the MCM complex. Required for sister chromatid cohesion.
總結(jié)	CJ119 is a carefully orchestrated process involving many proteins that assemble at origins of replication. Among these are the 6 proteins of the minichromosome maintenance (MCM) complex (e.g., MCM2; MIM 116945), which form a hexamer. Each MCM subunit performs an essential function in initiation and elongation of DNA replication. MCMBP can replace MCM2 in the MCM complex, thus forming an alternative MCM hexamer.
儲(chǔ)存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, IHC-P, FC, E
稀釋方法	WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50
圖像	Western blot analysis of CJ119 Antibody (N-term) (Cat. APR05350G) in MDA-MB231 cell line lysates (35ug/lane). CJ119 (arrow) was detected using the purified Pab. CJ119 Antibody (N-term) (Cat. APR05350G) immunohistochemistry analysis in formalin fixed and paraffin embedded human cervix carcinoma tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CJ119 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated. CJ119 Antibody (N-term) (Cat. APR05350G) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
說明書	Product Datasheet Protocol
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