Phospho-SEPARIN(S1126) Antibody

APR05152G Product Datasheet Protocol

貨號	貨期	規(guī)格 / 價格	詢價
APR05152G		100 μl / ￥4950

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安諾倫（北京）生物科技有限公司

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Phospho-SEPARIN(S1126) Antibody

品牌	Leading Biology	貨號	APR05152G
產(chǎn)品分類	Polyclonal Antibodies	研究領域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality Phospho-SEPARIN(S1126) Antibody.
分子量	233175 Da
細胞定位	Antigen Cellular Localization: Cytoplasm. Nucleus.
宿主	Rabbit
種屬反應性	Human
靶點	This SEPARIN Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S1126 of human SEPARIN.
亞型	Rabbit Ig
通用名	ESP1, KIAA0165
基因ID	9700
UniProt ID	Q14674
功能	Caspase-like protease, which plays a central role in the chromosome segregation by cleaving the SCC1/RAD21 subunit of the cohesin complex at the onset of anaphase. During most of the cell cycle, it is inactivated by different mechanisms.
總結	The metaphase-to-anaphase transition is the final discrete event in duplication and separation of the genetic material of the cell. Its timing is regulated by the activation of the anaphase-promoting complex (APC). In both budding and fission yeast, the degradation of the Pds1 or Cut2 protein, respectively, is required for the onset of sister chromatid separation. Both proteins are APC substrates. Pds1 and Cut2 proteins associate with the yeast separin proteins Esp1 and Cut1, respectively, and prevent the separins from promoting chromatid separation. Pds1 and Cut2 are also called anaphase inhibitors or securins
儲存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應用	DB, IHC-P, E
稀釋方法	DB~~1:500 IHC-P~~1:50~100
圖像	Dot blot analysis of anti-hSeparase-S801 Phospho-specific Pab (Cat. APR05152G) on nitrocellulose membrane. 50ng of nonphospho-peptide or phospho-peptide were adsorbed on their respective dots. Antibody working concentration was 0.5ug per ml. Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
說明書	Product Datasheet Protocol
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