品牌 |
Leading Biology |
貨號 |
APR05036G |
產(chǎn)品分類 |
Polyclonal Antibodies |
研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality ART3 Antibody (C-term).
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分子量 |
43923 Da
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細(xì)胞定位 |
Antigen Cellular Localization:
Cell membrane; Lipid-anchor, GPI-anchor.
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human
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免疫原 |
280-310 aa
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靶點(diǎn) |
This ART3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 280-310 amino acids from the C-terminal region of human ART3.
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亞型 |
Rabbit Ig
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通用名 |
TMART
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基因ID |
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UniProt ID |
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總結(jié) |
Mono-ADP-ribosylation involves the transfer of the ADP-ribose moiety from NAD+ to a specific amino acid in the target protein. The rodent mono-ADP-ribosyltransferase RT6 is a glycosylphosphatidylinositol (GPI)-anchored membrane protein specifically expressed at the cell surface of rat and mouse T lymphocytes. The predicted 367-amino acid human ART3 protein has an estimated molecular mass of 41.5 kD and contains a hydrophobic peptide signal at its N terminus, 3 consensus motifs specific to enzymes catalyzing ADP-ribose transfer, a hydrophobic C-terminal sequence characteristic of a GPI-anchored protein, a novel motif repeated 3 times at its C terminus, and 1 potential glycosylation site.1 The ART3 and rodent RT6 proteins share 35% amino acid identity.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, E
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稀釋方法 |
WB~~1:1000
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圖像 |
Anti-ART3 Antibody at 1:4000 dilution + human testis lysate
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 44 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot analysis of ART3 Antibody (C-term) in HL60 cell line lysates (25ug/lane). ART3(arrow) was detected using the purified Pab (4 ug/ml).
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說明書 |
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數(shù)量 |
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