All lanes : Anti-CCDC144A Antibody (C-Term) at 1:2000 dilution
Lane 1: human brain lysate
Lane 2: HL-60 whole cell lysate
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 165 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.