All lanes : Anti-WBP1 Antibody (C-Term) at 1:2000-1:4000 dilution
Lane 1: MCF-7 whole cell lysate
Lane 2: human liver lysate
Lane 3: human lung lysate
Lane 4: human placenta lysate
Lane 5: human kidney lysate
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 29 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.