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DKK1 Antibody

貨號(hào) 貨期 規(guī)格 / 價(jià)格 詢(xún)價(jià)
APR04794G 100 μl / ￥4950

DKK1 Antibody

品牌

 Leading Biology

貨號(hào)

 APR04794G

產(chǎn)品分類(lèi)

 Polyclonal Antibodies

研究領(lǐng)域

產(chǎn)品概述
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality DKK1 Antibody.

分子量
28672 Da

細(xì)胞定位
Antigen Cellular Localization: Secreted.

宿主
Rabbit

種屬反應(yīng)性
Human, Mouse

免疫原
1-266 aa

靶點(diǎn)
This DKK1 antibody is generated from a rabbit immunized with a recombinant protein of human DKK1.

亞型
Rabbit Ig

基因ID

UniProt ID

功能
Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.

總結(jié)
Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.

儲(chǔ)存條件
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

應(yīng)用
WB, IHC-P, FC, E

稀釋方法
FC~~1:25 IHC-P~~1:25 WB~~1:1000-1:4000

圖像

Overlay histogram showing U-2 OS cells stained with APR04794G (green line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (APR04794G, 1:25 dilution) for 60 min at 37?C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

APR04794G staining DKK1 in Human lung adenocarcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Anti-DKK1 Antibody at 1:1000 dilution + HepG2 whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 29 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

說(shuō)明書(shū)

數(shù)量

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