SNAI1 Antibody (N-term R8)

APR04615G Product Datasheet Protocol

貨號(hào)	貨期	規(guī)格 / 價(jià)格	詢(xún)價(jià)
APR04615G		100 μl / ￥4950

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SNAI1 Antibody (N-term R8)

品牌	Leading Biology	貨號(hào)	APR04615G
產(chǎn)品分類(lèi)	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality SNAI1 Antibody (N-term R8).
分子量	29083 Da
細(xì)胞定位	Antigen Cellular Localization: Nucleus. Cytoplasm. Note=Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) it is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs
宿主	Rabbit
種屬反應(yīng)性	Human
免疫原	1-30 aa
靶點(diǎn)	This SNAI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human SNAI1.
亞型	Rabbit Ig
通用名	SNAH
基因ID	6615
UniProt ID	O95863
功能	Involved in induction of the epithelial to mesenchymal transition (EMT), formation and maintenance of embryonic mesoderm, growth arrest, survival and cell migration. Binds to 3 E-boxes of the E-cadherin/CDH1 gene promoter and to the promoters of CLDN7 and KRT8 and, in association with histone demethylase KDM1A which it recruits to the promoters, causes a decrease in dimethylated H3K4 levels and represses transcription. Associates with EGR1 and SP1 to mediate tetradecanoyl phorbol acetate (TPA)-induced up- regulation of CDKN2B, possibly by binding to the CDKN2B promoter region 5'-TCACA-3. In addition, may also activate the CDKN2B promoter by itself.
總結(jié)	The Drosophila embryonic protein snail is a zinc finger transcriptional repressor which downregulates the expression of ectodermal genes within the mesoderm. The nuclear protein is structurally similar to the Drosophila snail protein, and is also thought to be critical for mesoderm formation in the developing embryo.
儲(chǔ)存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, E
稀釋方法	WB~~1:1000
圖像	Western blot analysis of lysates from Hela, MCF-7 cell line (from left to right), using SNAI1 Antibody (N-term R8)(APR04615G). APR04615G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane. Western blot analysis of mouse kidney tissue lysate, using SNAI1 Antibody (N-term R8)(APR04615G). APR04615G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane. Western blot analysis of lysates from Hela, SiHa cell line (from left to right), using SNAI1 Antibody (N-term R8)(APR04615G). APR04615G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
說(shuō)明書(shū)	Product Datasheet Protocol
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