RNF31 Antibody (C-term)

APR04414G Product Datasheet Protocol

貨號	貨期	規(guī)格 / 價格	詢價
APR04414G		100 μl / ￥4950

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安諾倫（北京）生物科技有限公司

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RNF31 Antibody (C-term)

品牌	Leading Biology	貨號	APR04414G
產品分類	Polyclonal Antibodies	研究領域
產品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality RNF31 Antibody (C-term).
分子量	119652 Da
細胞定位	Antigen Cellular Localization: Cytoplasm.
宿主	Rabbit
種屬反應性	Human
免疫原	955-983 aa
靶點	This RNF31 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 955-983 amino acids from the C-terminal region of human RNF31.
亞型	Rabbit Ig
通用名	ZIBRA
基因ID	55072
UniProt ID	Q96EP0
功能	E3 ubiquitin-protein ligase component of the LUBAC complex which conjugates linear ('Met-1'-linked) polyubiquitin chains to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF- induced cell death and thereby prevents inflammation. LUBAC is proposed to be recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Together with FAM105B/otulin, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis. Binds polyubiquitin of different linkage types.
總結	The protein encoded by this gene contains a RING finger, a motif present in a variety of functionally distinct proteins and known to be involved in protein-DNA and protein-protein interactions.
儲存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應用	WB, E
稀釋方法	WB~~1:1000
圖像	All lanes : Anti-RNF31 Antibody (C-term) at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: CCRF-CEM whole cell lysate Lane 3: Hela whole cell lysate Lane 4: Jurkat whole cell lysate Lane 5: MDA-MB-453 whole cell lysate Lane 6: MCF-7 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 58 kDa Blocking/Dilution buffer: 5% NFDM/TBST. All lanes : Anti-RNF31 Antibody (C-term) at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: CCRF-CEM whole cell lysate Lane 3: Hela whole cell lysate Lane 4: Jurkat whole cell lysate Lane 5: MDA-MB-453 whole cell lysate Lane 6: MCF-7 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 58 kDa Blocking/Dilution buffer: 5% NFDM/TBST. All lanes : Anti-RNF31 Antibody (C-term) at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: CEM whole cell lysate Lane 3: Hela whole cell lysate Lane 4: HepG2 whole cell lysate Lane 5: Jurkat whole cell lysate Lane 6: MCF-7 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 120 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
說明書	Product Datasheet Protocol
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