品牌 |
Leading Biology |
貨號 |
APR04159G |
產品分類 |
Polyclonal Antibodies |
研究領域 |
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產品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality F9 Antibody (Center).
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分子量 |
51778 Da
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細胞定位 |
Antigen Cellular Localization:
Secreted.
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宿主 |
Rabbit
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種屬反應性 |
Human
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免疫原 |
266-295 aa
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靶點 |
This F9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 266-295 amino acids from the Central region of human F9.
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亞型 |
Rabbit Ig
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基因ID |
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UniProt ID |
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功能 |
Factor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca(2+) ions, phospholipids, and factor VIIIa.
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總結 |
This gene encodes vitamin K-dependent coagulation factorIX that circulates in the blood as an inactive zymogen. This factoris converted to an active form by factor XIa, which excises theactivation peptide and thus generates a heavy chain and a lightchain held together by one or more disulfide bonds. The role ofthis activated factor IX in the blood coagulation cascade is toactivate factor X to its active form through interactions with Ca+2ions, membrane phospholipids, and factor VIII. Alterations of thisgene, including point mutations, insertions and deletions, causefactor IX deficiency, which is a recessive X-linked disorder, alsocalled hemophilia B or Christmas disease.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應用 |
WB, E
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稀釋方法 |
WB~~1:1000
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圖像 |
All lanes : Anti-F9 Antibody (Center) at 1:2000 dilution
Lane 1: 293T/17 whole cell lysate
Lane 2: A431 whole cell lysate
Lane 3: HepG2 whole cell lysate
Lane 4: K562 whole cell lysate
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 51 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
F9 Antibody (Center) (Cat. APR04159G) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the F9 antibody detected the F9 protein (arrow).
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說明書 |
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數(shù)量 |
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