ATF3 Antibody (C-term)

APR04118G Product Datasheet Protocol

貨號(hào)	貨期	規(guī)格 / 價(jià)格	詢價(jià)
APR04118G		100 μl / ￥4950

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ATF3 Antibody (C-term)

品牌	Leading Biology	貨號(hào)	APR04118G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality ATF3 Antibody (C-term).
分子量	20576 Da
細(xì)胞定位	Antigen Cellular Localization: Nucleus {ECO:0000255\|PROSITE- ProRule:PRU00978, ECO:0000269\|PubMed:12034827}
宿主	Rabbit
種屬反應(yīng)性	Human
免疫原	140-168 aa
靶點(diǎn)	This ATF3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 140-168 amino acids from the C-terminal region of human ATF3.
亞型	Rabbit Ig
基因ID	467
UniProt ID	P18847
功能	This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. Isoform 2 activates transcription presumably by sequestering inhibitory cofactors away from the promoters.
總結(jié)	Activating transcription factor 3 is a member of themammalian activation transcription factor/cAMP responsiveelement-binding (CREB) protein family of transcription factors.Multiple transcript variants encoding two different isoforms havebeen found for this gene. The longer isoform represses rather thanactivates transcription from promoters with ATF binding elements.The shorter isoform (deltaZip2) lacks the leucine zipperprotein-dimerization motif and does not bind to DNA, and itstimulates transcription presumably by sequestering inhibitoryco-factors away from the promoter. It is possible that alternativesplicing of the ATF3 gene may be physiologically important in theregulation of target genes.
儲(chǔ)存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, FC, E
稀釋方法	WB~~1:1000 FC~~1:10~50
圖像	ATF3 Antibody (C-term) (Cat. APR04118G) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the ATF3 antibody detected the ATF3 protein (arrow). Western blot analysis of ATF3 (arrow) using rabbit polyclonal ATF3 Antibody (C-term) (Cat. APR04118G). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ATF3 gene. ATF3 Antibody (C-term) (Cat. APR04118G) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
說(shuō)明書	Product Datasheet Protocol
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