品牌 |
Leading Biology | 貨號(hào) |
APR03722G |
產(chǎn)品分類(lèi) |
Polyclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality CA1 Antibody (N-term).
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分子量 |
28870 Da
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細(xì)胞定位 |
Antigen Cellular Localization:
Cytoplasm.
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human
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免疫原 |
60-90 aa
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靶點(diǎn) |
This CA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 60-90 amino acids from the N-terminal region of human CA1.
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亞型 |
Rabbit Ig
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基因ID |
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UniProt ID |
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功能 |
Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea.
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總結(jié) |
Carbonic anhydrases (CAs) are a large family of zincmetalloenzymes that catalyze the reversible hydration of carbondioxide. They participate in a variety of biological processes,including respiration, calcification, acid-base balance, boneresorption, and the formation of aqueous humor, cerebrospinalfluid, saliva, and gastric acid. They show extensive diversity intissue distribution and in their subcellular localization. CA1 isclosely linked to CA2 and CA3 genes on chromosome 8, and it encodesa cytosolic protein which is found at the highest level inerythrocytes. Variants of this gene have been described in somepopulations. Multiple alternatively spliced variants, encoding thesame protein, have been identified. Transcript variants of CA1utilizing alternative polyA_sites have been described inliterature.
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儲(chǔ)存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, IHC-P, FC, E
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稀釋方法 |
WB~~1:1000
IHC-P~~1:10~50
FC~~1:10~50
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圖像 |
Western blot analysis of CA1 (arrow) using rabbit polyclonal CA1 Antibody (N-term) (Cat. APR03722G). HEK293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CA1 gene. CA1 Antibody (N-term) (Cat. APR03722G)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CA1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated. CA1 Antibody (N-term) (Cat. APR03722G) flow cytometric analysis of HEK293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
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說(shuō)明書(shū) |
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數(shù)量 |
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