總結(jié) |
The receptors on T cells consist of immunoglobulin like integral membrane glycoproteins containing 2 polypeptide subunits, alpha and beta, of similar molecular weight, 40 to 55 kD in the human. Like the immunoglobulins of the B cells, each T cell receptor subunit has, external to the cell membrane, an N terminal variable domain and a C terminal constant domain.T cell receptors recognise foreign antigens which have been processed as small peptides and bound to major histocompatibility complex molecules at the surface of antigen presenting cells. Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. In a single cell, the T cell receptor loci are rearranged and expressed in the order delta, gamma, beta, and alpha. If both delta and gamma rearrangements produce functional chains, the cell expresses delta and gamma. If not, the cell proceeds to rearrange the beta and alpha loci.
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圖像 |
All lanes : Anti-TCRB Antibody (Center)(APR03659G) at 1:1000 dilution
Lane 1: Jurkat whole cell lysates
Lane 2: MOLT-4 whole cell lysates
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 15 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-TCRB Antibody (Center)(APR03659G) at 1:500 dilution + MDA-MB-231 whole cell lysates
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 15 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-TCRB Antibody (Center) at 1:1000 dilution + human spleen lysates
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size :15 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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