PLAG1 Antibody (N-term)

APR03591G Product Datasheet Protocol

貨號(hào)	貨期	規(guī)格 / 價(jià)格	詢價(jià)
APR03591G		100 μl / ￥4950

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安諾倫（北京）生物科技有限公司

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PLAG1 Antibody (N-term)

品牌	Leading Biology	貨號(hào)	APR03591G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality PLAG1 Antibody (N-term).
分子量	55909 Da
細(xì)胞定位	Antigen Cellular Localization: Nucleus Note=Strong nucleolar localization when sumoylation is inhibited
宿主	Rabbit
種屬反應(yīng)性	Human
免疫原	8-35 aa
靶點(diǎn)	This PLAG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 8-35 amino acids from the N-terminal region of human PLAG1.
亞型	Rabbit Ig
基因ID	5324
UniProt ID	Q6DJT9
功能	Transcription factor whose activation results in up- regulation of target genes, such as IGFII, leading to uncontrolled cell proliferation: when overexpressed in cultured cells, higher proliferation rate and transformation are observed. Other target genes such as CRLF1, CRABP2, CRIP2, PIGF are strongly induced in cells with PLAG1 induction. Proto-oncogene whose ectopic expression can trigger the development of pleomorphic adenomas of the salivary gland and lipoblastomas. Overexpression is associated with up-regulation of IGFII, is frequently observed in hepatoblastoma, common primary liver tumor in childhood. Cooperates with CBFB-MYH11, a fusion gene important for myeloid leukemia.
總結(jié)	Pleomorphic adenoma gene 1 encodes a zinc finger proteinwith 2 putative nuclear localization signals. PLAG1, which isdevelopmentally regulated, has been shown to be consistentlyrearranged in pleomorphic adenomas of the salivary glands. PLAG1 isactivated by the reciprocal chromosomal translocations involving8q12 in a subset of salivary gland pleomorphic adenomas. Threetranscript variants encoding two different isoforms have been foundfor this gene.
儲(chǔ)存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, E
稀釋方法	WB~~1:1000
圖像	All lanes : Anti-PLAG1 Antibody (N-term) at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: U-87 MG whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 56 kDa Blocking/Dilution buffer: 5% NFDM/TBST. PLAG1 Antibody (N-term) (Cat. APR03591G) western blot analysis in NCI-H460,K562 cell line lysates (35ug/lane).This demonstrates the PLAG1 antibody detected the PLAG1 protein (arrow).
說(shuō)明書	Product Datasheet Protocol
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