MITF Antibody (N-term)

APR03575G Product Datasheet Protocol

貨號(hào)	貨期	規(guī)格 / 價(jià)格	詢價(jià)
APR03575G		100 μl / ￥4950

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MITF Antibody (N-term)

品牌	Leading Biology	貨號(hào)	APR03575G
產(chǎn)品分類	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality MITF Antibody (N-term).
分子量	58795 Da
細(xì)胞定位	Antigen Cellular Localization: Nucleus.
宿主	Rabbit
種屬反應(yīng)性	Human
免疫原	1-28 aa
靶點(diǎn)	This MITF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-28 amino acids from the N-terminal region of human MITF.
亞型	Rabbit Ig
通用名	BHLHE32
基因ID	4286
UniProt ID	O75030
功能	Transcription factor that regulates the expression of genes with essential roles in cell differentiation, proliferation and survival. Binds to symmetrical DNA sequences (E-boxes) (5'- CACGTG-3') found in the promoters of target genes, such as BCL2 and tyrosinase (TYR). Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1). Plays a critical role in the differentiation of various cell types, such as neural crest- derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.
總結(jié)	This gene encodes a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. It regulates the differentiation and development of melanocytes retinal pigment epithelium and is also responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified.
儲(chǔ)存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, IF, FC, E
稀釋方法	WB~~1:1000 IF~~1:10~50 FC~~1:10~50
圖像	Anti-MITF Antibody (N-term)at 1:2000 dilution + A375 whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 59 kDa Blocking/Dilution buffer: 5% NFDM/TBST. MITF Antibody (N-term) (Cat. APR03575G) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the MITF antibody detected the MITF protein (arrow). Confocal immunofluorescent analysis of MITF Antibody (N-term)( APR03575G) with hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
說明書	Product Datasheet Protocol
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