All lanes : Anti-CCDC81 Antibody (Center) at 1:500 dilution
Lane 1: Hela whole cell lysate
Lane 2: MDA-MB-453 whole cell lysate
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 76 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.