PSMB11 Antibody (C-term)

APR03554G Product Datasheet Protocol

貨號(hào)	貨期	規(guī)格 / 價(jià)格	詢價(jià)
APR03554G		100 μl / ￥4950

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安諾倫（北京）生物科技有限公司

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PSMB11 Antibody (C-term)

品牌	Leading Biology	貨號(hào)	APR03554G
產(chǎn)品分類(lèi)	Polyclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality PSMB11 Antibody (C-term).
分子量	32530 Da
細(xì)胞定位	Antigen Cellular Localization: Cytoplasm {ECO:0000255\|PROSITE- ProRule:PRU00809}. Nucleus
宿主	Rabbit
種屬反應(yīng)性	Human
免疫原	250-278 aa
靶點(diǎn)	This PSMB11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 250-278 amino acids from the C-terminal region of human PSMB11.
亞型	Rabbit Ig
基因ID	122706
UniProt ID	A5LHX3
功能	The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Incorporated instead of PSMB5 or PSMB8, this unit reduces the chymotrypsin-like activity of the proteasome (By similarity). Plays a pivotal role in development of CD8-positive T cells (By similarity).
總結(jié)	Proteasomes generate peptides that are presented by majorhistocompatibility complex (MHC) I molecules to other cells of theimmune system. Proteolysis is conducted by 20S proteasomes,complexes of 28 subunits arranged as a cylinder in 4heteroheptameric rings: alpha-1 to -7, beta-1 to -7, beta-1 to -7,and alpha-1 to -7. The catalytic subunits are beta-1 (PSMB6; MIM600307), beta-2 (PSMB7; MIM 604030), and beta-5 (PSMB5; MIM600306). Three additional subunits, beta-1i (PSMB9; MIM 177045),beta-2i (PSMB10; MIM 176847), and beta-5i (PSMB8; MIM 177046), areinduced by gamma-interferon (IFNG; MIM 147570) and arepreferentially incorporated into proteasomes to makeimmunoproteasomes. PSMB11, or beta-5t, is a catalytic subunitexpressed exclusively in cortical thymic epithelial cells (Murataet al., 2007 [PubMed 17540904]).
儲(chǔ)存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, IHC-P, E
稀釋方法	WB~~1:1000 IHC-P~~1:50~100
圖像	Anti-PSMB11 Antibody (C-term) at 1:1000 dilution + T47D whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 33 kDa Blocking/Dilution buffer: 5% NFDM/TBST. PSMB11 Antibody (C-term) (Cat. APR03554G)immunohistochemistry analysis in formalin fixed and paraffin embedded human thymoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PSMB11 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
說(shuō)明書(shū)	Product Datasheet Protocol
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