品牌 |
Leading Biology |
貨號 |
APR03535G |
產(chǎn)品分類 |
Polyclonal Antibodies |
研究領域 |
|
產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality RNF19B Antibody (Center).
|
分子量 |
77925 Da
|
細胞定位 |
Antigen Cellular Localization:
Cytoplasmic granule membrane; Multi-pass membrane protein
|
宿主 |
Rabbit
|
種屬反應性 |
Human
|
免疫原 |
369-398 aa
|
靶點 |
This RNF19B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 369-398 amino acids from the Central region of human RNF19B.
|
亞型 |
Rabbit Ig
|
通用名 |
IBRDC3, NKLAM
|
基因ID |
|
UniProt ID |
|
功能 |
E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates, such as UCKL1. Involved in the cytolytic activity of natural killer cells and cytotoxic T-cells.
|
總結 |
IBRDC3 is a cytolysis-associated transmembrane proteinexpressed in natural killer (NK) cells and T lymphocytes followingcytokine stimulation (Kozlowski et al., 1999 [PubMed10438909]).
|
儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
|
應用 |
WB, FC, E
|
稀釋方法 |
WB~~1:500
FC~~1:10~50
|
圖像 |
Anti-RNF19B Antibody (Center) at 1:500 dilution + THP-1 whole cell lysate
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 78 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
RNF19B Antibody (Center) (Cat. APR03535G) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
|
說明書 |
|
數(shù)量 |
|