All lanes : Anti-LRRC34 Antibody (Center) at 1:2000 dilution
Lane 1: HL-60 whole cell lysate
Lane 2: MOLT-4 whole cell lysate
Lane 3: NCI-H460 whole cell lysate
Lane 4: U-2 OS whole cell lysate
Lane 5: mouse heart lysate
Lane 6: rat testis lysate
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 46 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.