品牌 |
Leading Biology | 貨號(hào) |
APR03431G |
產(chǎn)品分類 |
Polyclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality RPS6 (Ser240/244) Antibody.
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分子量 |
28681 Da
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human
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免疫原 |
220-249 aa
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靶點(diǎn) |
This RPS6 (Ser240/244) Antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 220-249 amino acids from human RPS6.
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亞型 |
Rabbit Ig
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基因ID |
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UniProt ID |
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功能 |
May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.
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總結(jié) |
Ribosomes, the organelles that catalyze protein synthesis,consist of a small 40S subunit and a large 60S subunit. Togetherthese subunits are composed of 4 RNA species and approximately 80structurally distinct proteins. This gene encodes a cytoplasmicribosomal protein that is a component of the 40S subunit. Theprotein belongs to the S6E family of ribosomal proteins. It is themajor substrate of protein kinases in the ribosome, with subsets offive C-terminal serine residues phosphorylated by different proteinkinases. Phosphorylation is induced by a wide range of stimuli,including growth factors, tumor-promoting agents, and mitogens.Dephosphorylation occurs at growth arrest. The protein maycontribute to the control of cell growth and proliferation throughthe selective translation of particular classes of mRNA. As istypical for genes encoding ribosomal proteins, there are multipleprocessed pseudogenes of this gene dispersed through the genome.
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儲(chǔ)存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, IHC-P, FC, E
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稀釋方法 |
WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
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圖像 |
Western blot analysis of lysate from HeLa cell line, using RPS6 Antibody(Ser240/244)(Cat. APR03431G). APR03431G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane. RPS6 antibody (Ser240/244) (Cat. APR03431G) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RPS6 antibody (Ser240/244) for immunohistochemistry. Clinical relevance has not been evaluated. RPS6 Antibody (Ser240/244) (Cat. APR03431G) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
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說明書 |
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數(shù)量 |
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