PPT1 Antibody (C-term)

AMR09492G Product Datasheet Protocol

貨號(hào)	貨期	規(guī)格 / 價(jià)格	詢價(jià)
AMR09492G		100 μl / ￥4950

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安諾倫（北京）生物科技有限公司

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PPT1 Antibody (C-term)

品牌	Leading Biology	貨號(hào)	AMR09492G
產(chǎn)品分類	Monoclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality PPT1 antibody (C-term).
分子量	34193 Da
細(xì)胞定位	Antigen Cellular Localization: Lysosome.
宿主	Mouse
種屬反應(yīng)性	Human
免疫原	1-306 aa
靶點(diǎn)	This PPT1 antibody is generated from a mouse immunized with a full-length recombinant protein from human PPT1.
克隆號(hào)	1117CT11.2.1.4
亞型	IgG1
通用名	PPT
基因ID	5538
UniProt ID	P50897
功能	Removes thioester-linked fatty acyl groups such as palmitate from modified cysteine residues in proteins or peptides during lysosomal degradation. Prefers acyl chain lengths of 14 to 18 carbons.
總結(jié)	Removes thioester-linked fatty acyl groups such as palmitate from modified cysteine residues in proteins or peptides during lysosomal degradation. Prefers acyl chain lengths of 14 to 18 carbons.
形式	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
儲(chǔ)存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, FC, IHC-P, E
稀釋方法	WB~~1:1000 IHC-P~~1:25 FC~~1:25
別名	Palmitoyl-protein thioesterase 1, PPT-1, Palmitoyl-protein hydrolase 1, PPT1, PPT
圖像	All lanes : Anti-PPT1 Antibody (C-term) at 1:2000 dilution Lane 1: Hela whole cell lysates Lane 2: HepG2 whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 34 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Immunohistochemical analysis of paraffin-embedded H. cerebellum section using PPT1 Antibody (C-term)( AMR09492G). AMR09492G was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining. Flow cytometric analysis of HepG2 cells using PPT1 Antibody (C-term)(green, AMR09492G) compared to an isotype control of mouse IgG1(blue). AMR09492G was diluted at 1:25 dilution. An Alexa Fluor? 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.
說明書	Product Datasheet Protocol
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