SOX2 Antibody

APR11290G Product Datasheet Protocol

貨號	貨期	規(guī)格 / 價格	詢價
APR11290G		100 μl / ￥4950

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安諾倫（北京）生物科技有限公司

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SOX2 Antibody

品牌	Leading Biology	貨號	APR11290G
產品分類	Monoclonal Antibodies	研究領域
產品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality SOX2 antibody .
分子量	34310 Da
細胞定位	Antigen Cellular Localization: Nucleus.
宿主	Mouse
種屬反應性	Human
靶點	SOX2 recombinant protein is used to produce this monoclonal antibody.
克隆號	57CT23.3.4
亞型	IgG1
基因ID	6657
UniProt ID	P48431
功能	Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency. May function as a switch in neuronal development. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal (By similarity). Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation (By similarity).
總結	This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT).
形式	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
儲存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應用	WB, IHC-P, IF, FC, E
稀釋方法	WB~~1:200~2000 IF~~1:100 IHC-P~~1:50~100 FC~~1:10~50
別名	Transcription factor SOX-2, SOX2
圖像	Western blot analysis of lysate from NCCIT cell line, using SOX2 Antibody( APR11290G). APR11290G was diluted at 1:1000. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysate at 20μg. Western blot analysis of lysate from SOX2 protein, using SOX2 Antibody( APR11290G). APR11290G was diluted at 1:4000. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysate at 20μg. Fluorescent image of A549 cell stained with SOX2 Antibody( APR11290G/SG110310AA).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SOX2 primary antibody (1:25, 1 h at 37℃. For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37℃.Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃.SOX2 immunoreactivity is localized to Nucleus significantly.
說明書	Product Datasheet Protocol
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