UCHL1 Antibody

APR13913G Product Datasheet Protocol

貨號	貨期	規(guī)格 / 價格	詢價
APR13913G		100 μl / ￥4950

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UCHL1 Antibody

品牌	Leading Biology	貨號	APR13913G
產(chǎn)品分類	Monoclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality UCHL1 antibody.
分子量	24824 Da
細胞定位	Antigen Cellular Localization: Cytoplasm. Endoplasmic reticulum membrane; Lipid-anchor. Note=About 30% of total UCHL1 is associated with membranes in brain
宿主	Mouse
種屬反應(yīng)性	Human
靶點	This UCHL1 monoclonal antibody is generated from mouse immunized with UCHL1 recombinant protein.
克隆號	346CT2.2.1
亞型	IgG1,k
基因ID	7345
UniProt ID	P09936
功能	Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
總結(jié)	The protein encoded by this gene belongs to the peptidaseC12 family. This enzyme is a thiol protease that hydrolyzes apeptide bond at the C-terminal glycine of ubiquitin. This gene isspecifically expressed in the neurons and in cells of the diffuseneuroendocrine system. Mutations in this gene may be associatedwith Parkinson disease.
形式	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
儲存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, IF, IHC-P, E
稀釋方法	IHC-P~~1:25 IF~~1:25 WB~~1:120~1000
別名	Ubiquitin carboxyl-terminal hydrolase isozyme L1, UCH-L1, 6---, Neuron cytoplasmic protein 95, PGP 95, PGP95, Ubiquitin thioesterase L1, UCHL1
圖像	Immunohistochemical analysis of paraffin-embedded H. kidney section using UCHL1( APR13913G). APR13913G was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining. Fluorescent image of A549 cells stained with UCHL1 Antibody( APR13913G). APR13913G was diluted at 1:25 dilution. An Alexa Fluor? 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor? 555 conjugated with Phalloidin (red). Western blot analysis of lysates from U266, mouse Neuro-2a, rat PC-12, C6 cell line and mouse brain tissue lysate(from left to right), using UCHL1 Antibody( APR13913G). APR13913G was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35μg per lane.
說明書	Product Datasheet Protocol
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