NCK1 Antibody

APR08621G Product Datasheet Protocol

貨號(hào)	貨期	規(guī)格 / 價(jià)格	詢價(jià)
APR08621G		100 μl / ￥5450

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安諾倫（北京）生物科技有限公司

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NCK1 Antibody

品牌	Leading Biology	貨號(hào)	APR08621G
產(chǎn)品分類	Monoclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality NCK1 antibody.
分子量	47kDa
細(xì)胞定位	Antigen Cellular Localization: Cytoplasm. Endoplasmic reticulum. Nucleus. Note=Mostly cytoplasmic, but shuttles between the cytoplasm and the nucleus. Import into the nucleus requires the interaction with SOCS7. Predominantly nuclear following genotoxic stresses, such as UV irradiation, hydroxyurea or mitomycin C treatments
宿主	Mouse
種屬反應(yīng)性	Human, Monkey
克隆號(hào)	5B7
亞型	IgG1
通用名	NCK
基因ID	4690
UniProt ID	P16333
功能	Adapter protein which associates with tyrosine- phosphorylated growth factor receptors, such as KDR and PDGFRB, or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling. Modulates the activation of EIF2AK2/PKR by dsRNA. May play a role in cell adhesion and migration through interaction with ephrin receptors.
形式	Liquid. In PBS containing 0.02% sodium azide.
儲(chǔ)存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, IHC, FC, E
稀釋方法	E~~1/10000 WB~~1/500 - 1/2000 IHC~~1/200 - 1/1000 FC~~1/200 - 1/400
別名	Cytoplasmic protein NCK1, NCK adaptor protein 1, Nck-1, SH2/SH3 adaptor protein NCK-alpha, NCK1, NCK
圖像	Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng); Figure 1: Western blot analysis using NCK1 mAb against human NCK1 (AA: 203-371) recombinant protein. (Expected MW is 44.9 kDa) Figure 2: Western blot analysis using NCK1 mouse mAb against Jurkat (1), HeLa (2), HEK293 (3), A431 (4), K562 (5), and COS7 (6) cell lysate.
說明書	Product Datasheet Protocol
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