品牌 |
Leading Biology | 貨號 |
APR17765G |
產(chǎn)品分類 |
Monoclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality GATA3 antibody (Center).
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分子量 |
H=48 KDa
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細胞定位 |
Antigen Cellular Localization:
Nucleus.
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宿主 |
Mouse
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種屬反應(yīng)性 |
Human
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靶點 |
This GATA3 antibody is generated from a mouse immunized with a recombinant protein from human GATA3.
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基因ID |
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UniProt ID |
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功能 |
Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'. Required for the T-helper 2 (Th2) differentiation process following immune and inflammatory responses.
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總結(jié) |
Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'.
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形式 |
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, FC, IF
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稀釋方法 |
WB~~ 1:1000
FC~~1:25
IF~~1:25
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別名 |
Trans-acting T-cell-specific transcription factor GATA-3, GATA-binding factor 3, GATA3
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圖像 |
Western blot analysis of lysates from CEM, SH-SY5Y cell line (from left to right), using GATA3 Antibody (Center)(Cat. APR17765G). APR17765G was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Flow cytometric analysis of MCF-7 cells using GATA3 Antibody (Center)(green, APR17765G) compared to an isotype control of mouse IgG2b(blue). APR17765G was diluted at 1:25 dilution. An Alexa Fluor? 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody. Fluorescent image of MCF-7 cells stained with GATA3 Antibody (Center)( APR17765G). APR17765G was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor? 555 conjugated with Phalloidin (red). |
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說明書 |
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數(shù)量 |
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