RAB27B Antibody

AMM07459G Product Datasheet Protocol

貨號	貨期	規(guī)格 / 價格	詢價
AMM07459G		100 μl / ￥4950

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安諾倫（北京）生物科技有限公司

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RAB27B Antibody

品牌	Leading Biology	貨號	AMM07459G
產(chǎn)品分類	Monoclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality RAB27B antibody.
分子量	24608 Da
細(xì)胞定位	Antigen Cellular Localization: Membrane; Lipid-anchor.
宿主	Mouse
種屬反應(yīng)性	Human, Mouse
靶點	This RAB27B antibody is generated from a mouse immunized with a recombinant protein of human RAB27B.
克隆號	1596CT245.254.49
亞型	IgG1,k
基因ID	5874
UniProt ID	O00194
功能	May be involved in targeting uroplakins to urothelial apical membranes.
總結(jié)	May be involved in targeting uroplakins to urothelial apical membranes.
形式	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
儲存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	FC, WB, E
稀釋方法	FC~~1:25 WB~~1:2000
別名	Ras-related protein Rab-27B, C25KG, RAB27B
圖像	Overlay histogram showing A431 cells stained with AMM07459G(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AMM07459G, 1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight? 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40 min at 37?C. Isotype control antibody (blue line) was mouse IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. All lanes : Anti-RAB27B Antibody at 1:2000 dilution Lane 1: DU145 whole cell lysate Lane 2: mouse stomach lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 25 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
說明書	Product Datasheet Protocol
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