品牌 |
Leading Biology |
貨號 |
AMM06406G |
產品分類 |
Monoclonal Antibodies |
研究領域 |
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產品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality MKRN2 antibody.
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分子量 |
46940 Da
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宿主 |
Mouse
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種屬反應性 |
Human
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靶點 |
This MKRN2 antibody is generated from a mouse immunized with recombinant protein.
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克隆號 |
1556CT631.230.55.49
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亞型 |
IgG1,k
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通用名 |
RNF62
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基因ID |
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UniProt ID |
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功能 |
E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins.
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總結 |
E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins.
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形式 |
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應用 |
IHC-P, FC, WB, E
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稀釋方法 |
IHC-P~~1:25
FC~~1:25
WB~~1:4000
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別名 |
Probable E3 ubiquitin-protein ligase makorin-2, 632-, RING finger protein 62, MKRN2, RNF62
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圖像 |
AMM06406G staining MKRN2 in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Overlay histogram showing K562 cells stained with AMM06406G(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AMM06406G, 1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight? 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40 min at 37?C. Isotype control antibody (blue line) was mouse IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
All lanes : Anti-MKRN2 Antibody at 1:4000 dilution
Lane 1: Jurkat whole cell lysate
Lane 2: K562 whole cell lysate
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 47 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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說明書 |
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數量 |
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