VAMP8 Antibody (N-term)

APR13937G Product Datasheet Protocol

貨號	貨期	規(guī)格 / 價格	詢價
APR13937G		100 μl / ￥4950

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安諾倫（北京）生物科技有限公司

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VAMP8 Antibody (N-term)

品牌	Leading Biology	貨號	APR13937G
產(chǎn)品分類	Monoclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality VAMP8 antibody (N-term).
分子量	11438 Da
細胞定位	Antigen Cellular Localization: Lysosome membrane; Single-pass type IV membrane protein. Late endosome membrane; Single-pass type IV membrane protein Note=Perinuclear vesicular structures of the early and late endosomes, coated pits, and trans-Golgi. Sub-tight junctional domain in retinal pigment epithelium cells. Midbody region during cytokinesis. Lumenal oriented, apical membranes of nephric tubular cell. Cycles through the apical but not through the basolateral plasma membrane. Apical region of acinar cells; in zymogen granule membranes (By similarity).
宿主	Mouse
種屬反應(yīng)性	Human
靶點	This VAMP8 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 2~24 amino acids from the N-terminal region of human VAMP8.
克隆號	1414CT354.12.23.93
亞型	IgG1,k
基因ID	8673
UniProt ID	Q9BV40
功能	SNAREs, Soluble N-ethylmaleimide-sensitive factor- attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. VAMP8 is a SNARE involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane. Also required for dense-granule secretion in platelets. Plays also a role in regulated enzyme secretion in pancreatic acinar cells. Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells. Involved in the homotypic fusion of early and late endosomes.
總結(jié)	SNAREs, Soluble N-ethylmaleimide-sensitive factor- attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. VAMP8 is a SNARE involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane. Also required for dense-granule secretion in platelets. Plays also a role in regulated enzyme secretion in pancreatic acinar cells. Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells. Involved in the homotypic fusion of early and late endosomes.
形式	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
儲存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, IHC-P, E
稀釋方法	IHC-P~~1:25 WB~~1:1000-1:2000
別名	Vesicle-associated membrane protein 8, VAMP-8, Endobrevin, EDB, VAMP8
圖像	Immunohistochemical analysis of paraffin-embedded H. brain section using VAMP8 Antibody (N-term)( APR13937G). APR13937G was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining. Western blot analysis of lysates from Hela, THP-1, A431 cell line (from left to right), using VAMP8 Antibody (N-term)( APR13937G). APR13937G was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20μg per lane.
說明書	Product Datasheet Protocol
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