品牌 |
Leading Biology |
貨號(hào) |
APR10198G |
產(chǎn)品分類(lèi) |
Monoclonal Antibodies |
研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality SOD2 antibody.
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分子量 |
24722 Da
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細(xì)胞定位 |
Antigen Cellular Localization:
Mitochondrion matrix.
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宿主 |
Mouse
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種屬反應(yīng)性 |
Human
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靶點(diǎn) |
Purified His-tagged SOD protein(Fragment) was used to produced this monoclonal antibody.
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克隆號(hào) |
37CT127.5.11.6
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亞型 |
IgG1κ
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基因ID |
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UniProt ID |
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功能 |
Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.
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總結(jié) |
This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
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形式 |
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
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儲(chǔ)存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
IHC-P, WB, E
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稀釋方法 |
WB~~1:2000
IHC-P~~1:10~50
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別名 |
Superoxide dismutase [Mn], mitochondrial, SOD2
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圖像 |
All lanes : Anti-SD Antibody at 1:2000 dilution
Lane 1: Hela whole cell lysates
Lane 2: human brain lysates
Lane 3: human heart lysates
Lane 4: human liver lysates
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 25 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with SOD2 Monoclonal Antibody (Cat. APR10198G), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of anti-SOD2 Monoclonal Antibody (Cat. APR10198G) by SOD2-GST fusion protein (GST MW=26 kD. SOD2-GST fusion protein (arrow) was detected using the purified Mab. (1:2000)
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說(shuō)明書(shū) |
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數(shù)量 |
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