品牌 |
Leading Biology | 貨號(hào) |
APR12436G |
產(chǎn)品分類 |
Monoclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality INA antibody.
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分子量 |
55391 Da
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宿主 |
Mouse
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種屬反應(yīng)性 |
Human, Mouse
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免疫原 |
290-319 aa
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靶點(diǎn) |
This INA antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 290-319 amino acids from human INA.
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克隆號(hào) |
257CT7.1.2
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亞型 |
IgG1,k
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通用名 |
NEF5
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基因ID |
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UniProt ID |
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功能 |
Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NF-L to form the filamentous backbone to which NF-M and NF-H attach to form the cross-bridges.
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總結(jié) |
Neurofilaments are type IV intermediate filamentheteropolymers composed of light, medium, and heavy chains.Neurofilaments comprise the axoskeleton and they functionallymaintain the neuronal caliber. They may also play a role inintracellular transport to axons and dendrites. This gene is amember of the intermediate filament family and is involved in themorphogenesis of neurons.
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形式 |
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
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儲(chǔ)存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, IF, IHC-P, E
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稀釋方法 |
WB~~1:100~250
IF~~1:10~50
IHC-P~~1:10~50
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別名 |
Alpha-internexin, Alpha-Inx, 66 kDa neurofilament protein, NF-66, Neurofilament-66, Neurofilament 5, INA, NEF5
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圖像 |
Anti-INA Antibody at 1:1000 dilution + SH-SY5Y whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 55 kDa Blocking/Dilution buffer: 5% NFDM/TBST. INA Antibody (Cat. APR12436G) western blot analysis in mouse brain tissue lysates (35μg/lane).This demonstrates the INA antibody detected the INA protein (arrow). Confocal immunofluorescent analysis of INA Antibody ( APR12436G) with brain tissue followed by Alexa Fluor? 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue). |
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說(shuō)明書(shū) |
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數(shù)量 |
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