品牌 |
Leading Biology |
貨號 |
AMM05908G |
產(chǎn)品分類 |
Monoclonal Antibodies |
研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality GFAP antibody.
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分子量 |
49880 Da
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細胞定位 |
Antigen Cellular Localization:
Cytoplasm. Note=Associated with intermediate filaments
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宿主 |
Mouse
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種屬反應性 |
Human
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靶點 |
This GFAP monoclonal antibody is generated from mouse immunized with GFAP recombinant protein.
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克隆號 |
183CT3.1.5
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亞型 |
IgG2b,k
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基因ID |
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UniProt ID |
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功能 |
GFAP, a class-III intermediate filament, is a cell- specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
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總結(jié) |
This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
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形式 |
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應用 |
WB, IHC-P, IF, E
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稀釋方法 |
WB~~1:100~500
IHC-P~~1:50~100
IF~~1:10~50
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別名 |
Glial fibrillary acidic protein, GFAP, GFAP
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圖像 |
All lanes : Anti-GFAP Antibody at 1:4000 dilution
Lane 1: human brain lysate
Lane 2: human cerebellum lysate
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 50 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
GFAP Antibody (Cat. AMM05908G) western blot analysis in MCF-7 cell line lysates (35μg/lane).This demonstrates the GFAP antibody detected the GFAP protein (arrow).
GFAP Monoclonal antibody (Cat. AMM05908G) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjμgation of the secondary antibody and DAB staining. This data demonstrates the use of the GFAP Monoclonal antibody( Ascites) for immunohistochemistry. Clinical relevance has not been evaluated.
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說明書 |
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數(shù)量 |
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