SOX2 Antibody (Ascites)

APR14253G Product Datasheet Protocol

貨號(hào)	貨期	規(guī)格 / 價(jià)格	詢價(jià)
APR14253G		0.1 ml / ￥5450

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安諾倫（北京）生物科技有限公司

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SOX2 Antibody (Ascites)

品牌	Leading Biology	貨號(hào)	APR14253G
產(chǎn)品分類	Monoclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality SOX2 antibody (Ascites).
分子量	34310 Da
細(xì)胞定位	Antigen Cellular Localization: Nucleus.
宿主	Mouse
種屬反應(yīng)性	Human
靶點(diǎn)	Human SOX2 recombinant protein is used to produce this monoclonal antibody.
克隆號(hào)	57CT23.3.4
亞型	IgG1
基因ID	6657
UniProt ID	P48431
功能	Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency. May function as a switch in neuronal development. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal (By similarity). Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation (By similarity).
總結(jié)	This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT).
形式	Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
儲(chǔ)存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	IF, WB, FC, E
稀釋方法	IF~~1:200 WB~~1:200~2000 FC~~1:50~100
別名	Transcription factor SOX-2, SOX2
圖像	Fluorescent confocal image of an SY5Y cell stained with APR14253G SOX2 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with APR14253G SOX2 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (5.25 μM, 25 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 3 min). SOX2 immunoreactivity is localized exclusively to the nucleus. Western blot analysis of anti-SOX2 Monoclonal Antibody(Cat. APR14253G) by human SOX2 recombinant protein cell line lysate (35μg/lane). SOX2 (His tagged) (arrow) was detected using the purified Mab. Flow cytometric analysis of NCI-H460 cells using SOX2 Monoclonal Antibody (ascites)(bottom histogram) compared to a negative control cell (top histogram). PE-conjugated goat-anti-mouse secondary antibodies were used for the analysis.
說明書	Product Datasheet Protocol
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