Functional Myosin IIA (non-muscle) (heavy chain) Antibody, mAb (recombinant)

AMP00010G Product Datasheet Protocol

貨號	貨期	規(guī)格 / 價格	詢價
AMP00010G		100 μg / ￥5450

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Functional Myosin IIA (non-muscle) (heavy chain) Antibody, mAb (recombinant)

品牌	Leading Biology	貨號	AMP00010G
產(chǎn)品分類	Monoclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality Functional Myosin IIA (non-muscle) (heavy chain) Antibody, mAb (recombinant).
分子量	223044 Da
細胞定位	Antigen Cellular Localization: Cytoplasm, myofibril. Note=Thick filaments of the myofibrils
宿主	Purified From?HEK 293 Cell?culture Supernatant.
種屬反應(yīng)性	Human, Mouse, Rat, Drosophila
靶點	Recognizes human, mouse, rat and drosophila myosin IIA (heavy chain).
克隆號	SF9
亞型	Human IgG2λ
通用名	MYHSA2
基因ID	4620
UniProt ID	Q9UKX2
功能	Muscle contraction. Required for cytoskeleton organization (By similarity).
總結(jié)	anti-Myosin IIA (non-muscle), monoclonal antibody (recombinant) (SF9) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.
形式	Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.
儲存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	WB, ICC, E
圖像	Human Myosin IIA (non-muscle) (heavy chain) is detected by immunocytochemistry using anti-myosin IIA (non-muscle) (rec.) (SF9). Method:HeLa cells are grown in standard culture conditions, fixed with methanol, and incubated with anti-Myosin IIA (non-muscle) (rec.) (SF9) (1mg /ml in PBS-BSA). After incubation for 30 min at RT and several washes in PBS, cells are treated with a goat anti-human (Cy3) antibody for 30 min at RT, washed and mounted in Moewiol. Nuclei are stained with DAPI. Picture courtesy of Dr. Moutel, Dr. Franck Perez lab, Curie Institute, Paris.
說明書	Product Datasheet Protocol
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Functional Myosin IIA (non-muscle) (heavy chain) Antibody, mAb (recombinant)