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當(dāng)前位置：首頁(yè) > 抗原抗體、ELISA、WB > 一抗 > Monoclonal Antibodies > EEF1E1 Antibody

EEF1E1 Antibody

貨號(hào) 貨期 規(guī)格 / 價(jià)格 詢價(jià)
AMM04350G 100 μl / ￥4950

EEF1E1 Antibody

品牌

 Leading Biology

貨號(hào)

 AMM04350G

產(chǎn)品分類

 Monoclonal Antibodies

研究領(lǐng)域

產(chǎn)品概述
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality EEF1E1 Antibody.

分子量
H=20,16;M=20 KDa

細(xì)胞定位
Antigen Cellular Localization: Cytoplasm. Nucleus. Note=Cytoplasmic under growth arrest conditions. Translocated into the nucleus when growth resumes (S phase) and following DNA damage

宿主
Mouse

種屬反應(yīng)性
Human

免疫原
1-112 aa

靶點(diǎn)
This EEF1E1 antibody is generated from a mouse immunized with a recombinant protein of human EEF1E1.

通用名
AIMP3, P18

基因ID

UniProt ID

功能
Positive modulator of ATM response to DNA damage.

總結(jié)
Positive modulator of ATM response to DNA damage.

儲(chǔ)存條件
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

應(yīng)用
FC, IF, WB

稀釋方法
WB~~1:2000 FC~~1:25 IF~~1:25

圖像

All lanes : Anti-EEF1E1 Antibody at1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: A431 whole cell lysate Lane 3: A549 whole cell lysate Lane 4: Jurkat whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 20 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Overlay histogram showing Hela cells stained with AMM04350G (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AMM04350G, 1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight? 488 Conjugated Highly Cross-Adsorbed(NA168821)) at 1/400 dilution for 40 min at 37?C. Isotype control antibody (blue line) was mouse IgG2b (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling Pdx1 with AMM04350G at 1/25 dilution, followed by Dylight? 488-conjugated goat anti-mouse IgG (NA166821) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight? 554 Phalloidin (PD18466410) at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).

說(shuō)明書

數(shù)量

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