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當前位置:首頁 > 抗原抗體、ELISA、WB > 一抗 > Monoclonal Antibodies > Beta-Actin Antibody

Beta-Actin Antibody

貨號 貨期 規(guī)格 / 價格 詢價
AMM04349G 100 μl / ¥4950

Beta-Actin Antibody

品牌

Leading Biology

貨號

AMM04349G

產(chǎn)品分類

Monoclonal Antibodies

研究領域

產(chǎn)品概述

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality Beta-Actin Antibody.

分子量

H=42;R=42;M=42 KDa

細胞定位

Antigen Cellular Localization: Cytoplasm, cytoskeleton. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs

宿主

Mouse

種屬反應性

Human, Mouse

免疫原

1-375 aa

靶點

This ACTB Monoclonal antibody is generated from mouse immunized with ACTB recombinant protein.

基因ID

UniProt ID

功能

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

總結

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.

儲存條件

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

應用

WB

稀釋方法

FC~~1:25 IHC~~1:25 WB~~1:4000

圖像

Overlay histogram showing A431 cells stained with AMM04349G(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AMM04349G, 1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight? 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40 min at 37?C. Isotype control antibody (blue line) was mouse IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

AMM04349G staining ACTB in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

All lanes : Anti-ACTB Antibody at 1:4000 dilution Lane 1: A431 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: C2C12 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 42 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

說明書

數(shù)量

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