品牌 |
Leading Biology | 貨號(hào) |
AMM04344G |
產(chǎn)品分類 |
Monoclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality CD74 Antibody.
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分子量 |
H=34,26,18 KDa
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細(xì)胞定位 |
Antigen Cellular Localization:
Cell membrane; Single-pass type II membrane protein. Endoplasmic reticulum membrane. Golgi apparatus, trans-Golgi network. Endosome Lysosome. Note=Transits through a number of intracellular compartments in the endocytic pathway. It can either undergo proteolysis or reach the cell membrane
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宿主 |
Mouse
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種屬反應(yīng)性 |
Human
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免疫原 |
1-232 aa
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靶點(diǎn) |
This antibody is generated from a mouse immunized with a recombinant protein.
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通用名 |
DHLAG
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基因ID |
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UniProt ID |
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功能 |
Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF.
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總結(jié) |
Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF.
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儲(chǔ)存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
FC, WB
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稀釋方法 |
WB~~1:1000
FC~~1:25
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圖像 |
All lanes : Anti-CD74 Antibody at 1:1000 dilution Lane 1: Daudi whole cell lysates Lane 2: Raji whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Mouse IgG (H+L),Peroxidase conjugated at 1/5,000 dilution Predicted band size : 34 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Flow cytometric analysis of Raji cells using CD74(green, AMM04344G) compared to an isotype control of mouse IgG2b(blue). AW5502 was diluted at 1:25 dilution. An Alexa Fluor? 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody. |
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說(shuō)明書(shū) |
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數(shù)量 |
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