CD73(NT5E) Antibody (C-term)

AMM02468G Product Datasheet Protocol

貨號	貨期	規(guī)格 / 價格	詢價
AMM02468G		100μl / ￥4950

大規(guī)格詢價

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品牌	Leading Biology	貨號	AMM02468G
產(chǎn)品分類	Monoclonal Antibodies	研究領(lǐng)域
產(chǎn)品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality CD73(NT5E) Antibody (C-term).
分子量	63368 Da
細胞定位	Antigen Cellular Localization: Cell membrane; Lipid-anchor, GPI-anchor.
宿主	Mouse
種屬反應(yīng)性	Human
靶點	Purified His-tagged CD73(NT5E) protein was used to produced this monoclonal antibody.
克隆號	974CT7.5.3
亞型	IgG1
通用名	NT5, NTE
基因ID	4907
UniProt ID	P21589
功能	Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities.
總結(jié)	Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.
儲存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用	IHC-P, WB, E
稀釋方法	IHC-P~~1:25 WB~~1:5000
圖像	AM2223A staining CD73 (NT5E) in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody. AM2223A staining CD73 (NT5E) in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody. Anti-CD73 (NT5E) Antibody (C-term) at 1:4000 dilution + MDA-MB-231 whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 63 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
說明書	Product Datasheet Protocol
數(shù)量

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