品牌 |
Leading Biology | 貨號 |
AMM02320G |
產(chǎn)品分類 |
Monoclonal Antibodies | 研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality Beta-actin Antibody.
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分子量 |
41737 Da
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細(xì)胞定位 |
Antigen Cellular Localization:
Cytoplasm, cytoskeleton. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs
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宿主 |
Mouse
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種屬反應(yīng)性 |
Human, Mouse, Rat
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免疫原 |
Unknown aa
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靶點(diǎn) |
ACTB recombinant protein is used to produce this monoclonal antibody.
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克隆號 |
8H10D10
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亞型 |
IgG
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基因ID |
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UniProt ID |
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功能 |
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
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總結(jié) |
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, IHC-P, E
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稀釋方法 |
WB~~1:1000
IHC-P~~1:10~50
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圖像 |
Western blot analysis of lysates from HepG2, HL-60 cell line (from left to right), using Beta-actin Antibody(AMM02320G). AMM02320G was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20μg per lane. Immunohistochemical analysis of paraffin-embedded H. spleen section using Beta-actin Antibody( AMM02320G). AMM02320G was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining. Western blot analysis of lysates from HepG2, HL-60, mouse NIH/3T3 cell line, mouse cerebellum and rat stomach tissue lysate, CHO, COS-7 cell line lysate(from left to right), using Beta-actin Antibody(AMM02320G). AMM02320G was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35μg per lane. |
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說明書 |
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數(shù)量 |
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